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Biological rhythms in the deep-sea hydrothermal mussel Bathymodiolus azoricus ArchiMer
Mat, Audrey; Sarrazin, Jozee; Markov, Gabriel V.; Apremont, Vincent; Dubreuil, Christine; Eché, Camille; Fabioux, Caroline; Klopp, Christophe; Sarradin, Pierre-marie; Tanguy, Arnaud; Huvet, Arnaud; Matabos, Marjolaine.
Biological rhythms are a fundamental property of life. The deep ocean covers 66% of our planet surface and is one of the largest biomes. The deep sea has long been considered as an arrhythmic environment because sunlight is totally absent below 1,000 m depth. In the present study, we have sequenced the temporal transcriptomes of a deep-sea species, the ecosystem-structuring vent mussel Bathymodiolus azoricus. We reveal that tidal cycles predominate in the transcriptome and physiology of mussels fixed directly at hydrothermal vents at 1,688 m depth at the Mid-Atlantic Ridge, whereas daily cycles prevail in mussels sampled after laboratory acclimation. We identify B. azoricus canonical circadian clock genes, and show that oscillations observed in deep-sea...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00640/75236/75385.pdf
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Identification, Characterization, and Diel Pattern of Expression of Canonical Clock Genes in Nephrops norvegicus (Crustacea: Decapoda) Eyestalk ArchiMer
Sbragaglia, Valerio; Lamanna, Francesco; Mat, Audrey; Rotllant, Guiomar; Joly, Silvia; Ketmaier, Valerio; De La Iglesia, Horacio O.; Aguzzi, Jacopo.
The Norway lobster, Nephrops norvegicus, is a burrowing decapod with a rhythmic burrow emergence (24 h) governed by the circadian system. It is an important resource for European fisheries and its behavior deeply affects its availability. The current knowledge of Nephrops circadian biology is phenomenological as it is currently the case for almost all crustaceans. In attempt to elucidate the putative molecular mechanisms underlying circadian gene regulation in Nephrops, we used a transcriptomics approach on cDNA extracted from the eyestalk, a structure playing a crucial role in controlling behavior of decapods. We studied 14 male lobsters under 12-12 light-darkness blue light cycle. We used the Hiseq 2000 Illumina platform to sequence two eyestalk...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00483/59476/62314.pdf
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Influence of temperature on daily locomotor activity in the crab Uca pugilator ArchiMer
Mat, Audrey; Dunster, Gideon P.; Sbragaglia, Valerio; Aguzzi, Jacopo; De La Iglesia, Horacio O..
Animals living in the intertidal zone are exposed to prominent temperature changes. To cope with the energetic demands of environmental thermal challenges, ectotherms rely mainly on behavioral responses, which may change depending on the time of the day and seasonally. Here, we analyze how temperature shapes crabs’ behavior at 2 different times of the year and show that a transition from constant cold (13.5°C) to constant warm (17.5°C) water temperature leads to increased locomotor activity levels throughout the day in fiddler crabs (Uca pugilator) collected during the summer. In contrast, the same transition in environmental temperature leads to a decrease in the amplitude of the daily locomotor activity rhythm in crabs collected during the winter. In...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00384/49507/50001.pdf
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